The Sclerostin Polyclonal Antibody (CAB8213) is a valuable tool for researchers studying the protein sclerostin, a key regulator of bone formation and mineralization. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in various applications, including Western blot and immunohistochemistry.Sclerostin is an important factor in the Wnt signaling pathway, which plays a crucial role in bone development and homeostasis. Dysregulation of sclerostin is associated with conditions such as osteoporosis and bone fractures, making it a promising target for therapeutic interventions.
By binding to sclerostin in cell samples, this antibody enables researchers to investigate the expression and function of this protein in different biological contexts. Whether studying normal bone remodeling or pathological conditions affecting bone health, the Sclerostin Polyclonal Antibody is a valuable tool for gaining insights into the mechanisms underlying skeletal disorders.
Product Name:
SOST Rabbit Polyclonal Antibody
SKU:
CAB8213
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 24-213 of human SOST (NP_079513.1).
Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
Purification Method:
Affinity purification
Gene ID:
50964
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SOST using SOST Rabbit pAb(CAB8213) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 2s.
