The SCIMP Antibody (PAC060156) is a polyclonal antibody designed for research involving SCIMP, a key player in immune signaling pathways. This antibody, generated in rabbits, is highly specific for human samples and validated for Western blot applications. By binding to the SCIMP protein, this antibody enables accurate detection and analysis in various cell types, making it an excellent tool for studies in immunology and inflammatory diseases.SCIMP, also known as signaling adaptor protein, is involved in mediating immune responses and has been implicated in various diseases including cancer and autoimmune disorders.
Understanding the function of SCIMP is crucial for unraveling its role in immune regulation and developing targeted therapies. The SCIMP Antibody offers researchers a reliable tool for investigating the role of SCIMP in immune signaling pathways and its potential as a therapeutic target.Overall, the SCIMP Antibody is a valuable resource for researchers studying immune regulation, inflammation, and disease pathogenesis. Its high specificity and reliability make it an essential tool for advancing our knowledge of immune signaling and developing new therapies for immune-related disorders.
IHC image of PACO60156 diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO60156 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Lipid tetraspanin-associated transmembrane adapter/mediator involved in major histocompatibility complex (MHC) class II signaling transduction. Required in generating the calcium response and enhancing ERK activity upon MHC-II stimulation.
Synonyms:
SLP adapter and CSK-interacting membrane protein (SLP65/SLP76, Csk-interacting membrane protein), SCIMP, C17orf87
UniProt Protein Function:
UNQ5783: Lipid tetraspanin-associated transmembrane adapter/mediator involved in major histocompatibility complex (MHC) class II signaling transduction. Required in generating the calcium response and enhancing ERK activity upon MHC-II stimulation. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 17p13.2Cellular Component: immunological synapse; integral to membrane; membraneMolecular Function: protein bindingBiological Process: meiosis I
UniProt Protein Details:
NCBI Summary:
This gene encodes a transmembrane adaptor protein that is expressed in antigen-presenting cells and is localized in the immunologic synapse. The encoded protein is involved in major histocompatibility complex class II signal transduction and immune synapse formation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012]
