SAs (Sulfonamides of 3-in-1 ) ELISA Kit (FSES0028)

SKU:

FSES0028

Product Type:

ELISA Kit

Applications:

ELISA

Research Area:

Food Science

Description

Description

SAs (Sulfonamides of 3-in-1 ) ELISA Kit

Kit Name:

SAs (Sulfonamides of 3-in-1 ) ELISA Kit

Kit Size:

96 Assays

Test Principle:

This kit uses Competitive-ELISA as the method. It can detect Sulfonamides of 3-in-1 (SAs) in samples, such as muscle, honey, milk and feed. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, SAS in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-SAS antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of SAS. The concentration of SAS in the samples can be calculated by comparing the OD of the samples to the standard curve.

Sensitivity:

0.1 ppb (ng/mL)

Reaction Mode:

25°C, 45 min~15 min

Detection Limit:

Muscle (method 1) ---0.1 ppb; Muscle (method 2) ---1 ppb; Milk---2 ppb; Serum, Urine, Eggs---0.4 ppb; Honey---0.1 ppb; Feed---4 ppb.

Cross Reactivity:

Sulfamethoxazole (SMZ) --- 100%; Sulfamonomethoxine (SMM) --- 67%;Sulfadiazine (SD/SDZ) ---33%

Sample Recovery Rate:

Muscle, Honey, Eggs---85±25%; Urine, Milk, Serum, Feed---80±25%

Kits Components:

Item	Specifications
ELISA Microtiter plate	96 wells
Standard Liquid	1 mL each (0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb)
HRP Conjugate	5.5 mL
Antibody Working Solution	5.5 mL
Substrate Reagent A	6 mL
Substrate Reagent B	6 mL
Stop Solution	6 mL
20 x Concentrated Wash Buffer	40 mL
2 x Reconstitution Buffer	50 mL
Plate Sealer	3 pieces
Sealed Bag	1 piece
Manual	1 copy

Instruments:

Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01g).

Micropipette:

Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).

Reagents:

Ethyl acetate, N-hexane, Acetonitrile, Na2HPO412H2O, NaOH, Concentrated HCl, NaH2PO42H2O.

Storage And Expiry Date:

Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.