The SARS-CoV Spike RBD Monoclonal Antibody (CAB21181) is a specialized antibody designed for research involving the Spike Receptor Binding Domain (RBD) of the SARS-CoV virus. This monoclonal antibody, produced through advanced technology, is highly specific and sensitive for detecting the SARS-CoV Spike RBD protein in various biological samples.The SARS-CoV Spike RBD is a critical component of the virus responsible for binding to host cell receptors, facilitating viral entry, and initiating infection. By targeting the Spike RBD, researchers can better understand the mechanisms of SARS-CoV infection and develop strategies for diagnostic testing and vaccine development.
This monoclonal antibody is validated for use in a range of applications, including Western blotting, immunofluorescence, and immunohistochemistry, making it a valuable tool for studying the SARS-CoV virus and its interactions with host cells. Its high specificity and sensitivity ensure reliable and accurate results, making it ideal for research projects focused on developing antiviral therapies and understanding the immune response to SARS-CoV infection.
Product Name:
SARS-CoV Spike RBD Monoclonal Antibody
SKU:
CAB21181
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
SARS-CoV
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of coronavirus Spike RBD (NP_828851.1).
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Purification Method:
Affinity purification
Gene ID:
1489668
Clone Number:
ARC52567
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using SARS-CoV Spike RBD Rabbit mAb (CAB21181) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
![Anti-SARS-CoV-2 Spike RBD [2355]](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214681/569737/anti-sars-cov-2-spike-rbd-2355__83998.1673669692.jpg?c=2 "Anti-SARS-CoV-2 Spike RBD [2355]")
