The SARS-CoV-2 Spike RBD N501Y Monoclonal Antibody (CAB21254) offered by AssayGenie is a valuable tool for researchers studying the SARS-CoV-2 virus and its variants. This monoclonal antibody specifically targets the Spike protein receptor binding domain (RBD) with the N501Y mutation, which has been associated with increased transmissibility.Developed using cutting-edge technology, this antibody is highly specific and sensitive, allowing for accurate detection and analysis of the SARS-CoV-2 Spike protein in various sample types. It is validated for use in a variety of applications, including ELISA, Western blot, and immunofluorescence, making it versatile for researchers in the fields of virology, infectious diseases, and vaccine development.
Understanding the behavior of the Spike protein, especially with the N501Y mutation, is crucial for developing effective diagnostic tools, vaccines, and therapeutics against SARS-CoV-2. With the SARS-CoV-2 Spike RBD N501Y Monoclonal Antibody (CAB21254), researchers have a reliable tool to advance their research and contribute to the global effort in combating the COVID-19 pandemic.
Product Name:
SARS-CoV-2 Spike RBD (N501Y) Monoclonal Antibody
SKU:
CAB21254
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
SARS-CoV-2
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 450-550 of coronavirus SARS-CoV-2 Spike RBD (N501Y) (YP_009724390.1).
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Purification Method:
Affinity purification
Gene ID:
43740568
Clone Number:
ARC52673
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using SARS-CoV-2 Spike RBD (N501Y) antibody (CAB21254) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
