null

SARS-CoV-2 NSP12 Recombinant Protein (RPES7053)

SKU:
RPES7053
Product Type:
Recombinant Protein
Species:
Virus
$875

Description

Product Name:SARS-CoV-2 NSP12 Recombinant Protein
Product Code:RPES7053
Size:50µg
Species:SARS-CoV-2
Expression Host:E.coli
Synonyms:SARS-CoV 2 nsp12,RNA-directed RNA polymerase,RdRp
Mol Mass:64kDa
AP Mol Mass:60 kDa
Tag:N-Gst
Purity:> 90 % as determined by reducing SDS-PAGE.
Endotoxin Level:Please contact us for more information.
Bio Activity:Testing in progress
Sequence:Asp4891-Val5212
Accession:QHD43415.1
Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution:Please refer to the printed manual for detailed information.
Background:The RdRp of SARS-CoV-2 is composed of a catalytic subunit known as nsp12 as well as two accessory subunits, nsp8 and nsp7. The structure of this RdRp has recently been reported; it is highly similar to the RdRp of SARS-CoV, a zoonotic coronavirus that spread into the human population in 2002. The nsp12 subunit contains an N-terminal nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain, an interface domain and a C-terminal RdRp domain. The RdRp domain resembles a right hand, comprising the fingers, palm and thumb subdomains that are found in all single-subunit polymerases. Subunits nsp7 and nsp8 bind to the thumb, and an additional copy of nsp8 binds to the fingers domain. Structural information is also available for nsp8–nsp7 complexes.

Related Products