The SAFB Polyclonal Antibody (CAB15721) is a crucial tool for researchers studying the SAFB protein, a key player in gene expression regulation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to SAFB, the antibody facilitates the detection and analysis of this protein in various cell types, making it perfect for investigations in molecular biology and cancer research.SAFB, also known as scaffold attachment factor B, is involved in various cellular processes, including chromatin organization and transcriptional regulation.
Its role in gene expression makes it a valuable target for studies exploring diseases such as cancer, neurological disorders, and developmental abnormalities. Understanding the functions of SAFB is essential for developing targeted therapies that can modulate gene expression and potentially treat a range of conditions.
Product Name:
SAFB Rabbit Polyclonal Antibody
SKU:
CAB15721
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human SAFB (NP_002958.2).
This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6294
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using SAFB antibody (CAB15721) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 20s.
