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S100A6 Polyclonal Antibody (CAB21819)

SKU:
CAB21819
€129
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:S100A6 Polyclonal Antibody
SKU:CAB21819
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6(NP_055439.1).
Sequence:MACP LDQA IGLL VAIF HKYS GREG DKHT LSKK ELKE LIQK ELTI GSKL QDAE IARL MEDL DRNK DQEV NFQE YVTF LGAL ALIY NEAL KG
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:2A9; PRA; 5B10; CABP; CACY; S10A6; S100A6
Positive Sample:U-251 MG,,HeLa
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus envelope, Peripheral membrane protein.
Calculated MW:10kDa
Observed MW:10kDa

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.

Purification Method:Affinity purification
Gene ID:6277
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from U-251 MG cells using S100A6 Rabbit pAb(CAB21819) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

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