null

S.Enteritidis OmpA Recombinant Protein (RPPB4162)

SKU:
RPPB4162
Product Type:
Recombinant Protein
Species:
Salmonella enteritidis
Research Area:
Microbiology
€1,079
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:S.Enteritidis OmpA Recombinant Protein
Product Code:RPPB4162
Size:0.5mg
Species:S.Enteritidis
Target:OmpA
Synonyms:Outer Membrane Protein-A, OmpA.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:PBS.
Stability:OmpA S.Enteritidis Recombinant although stable at 4°C for 1 week, should be stored below -18°C.�Please prevent freeze thaw cycles.
Purity:Protein is >95% pure as determined by 10% PAGE (coomassie staining).

The OmpA protein is one of the main outer-membrane proteins of a large array of Gram-negative bacteria such as A.salmonicida, Shigella dysenteriae and E.coli.OmpA�s major physiological functions include maintenance of the structural integrity and morphology of the cells and porin activity, as well as a role in conjugation and bacteriophage binding.Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease.Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein.The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E.coli BL21(DE3). The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein.Recombinant A-protein was compared by biochemical, immunological and molecular methods with the A-protein isolated from atypical A.salmonicida bacterial cells by the glycine and the membrane extraction methods.

The Recombinant �Salmonella Enteritidis Outer Membrane Protein A, E.Coli derived, 330 amino acids, contains the ompA immunodominant regions. The protein is fused to a His tag at C-terminal and purified by standard chromatography techniques.

Related Products