RUNX3 Transcription Factor Activity Assay
- SKU:
- TFAB00075
- Product Type:
- ELISA Kit
- ELISA Type:
- Transcription Factor Assay
- Analytes:
- RUNX3
- Reactivity:
- Human
- Mouse
- Rat
Description
| Product Name: | RUNX3 Transcription Factor Activity Assay |
| Product Code: | TFAB00075 |
| Target: | RUNX3 |
| Synonyms: | Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3, CBF-alpha-3, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEA2-alpha C, PEBP2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie RUNX3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 1p36 Cellular Component: cytoplasm; nuclear chromatin; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: chondrocyte differentiation; hemopoiesis; negative regulation of cell cycle; negative regulation of epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; ossification; peripheral nervous system neuron development; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cell differentiation; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q13761 |
| NCBI GenInfo Identifier: | 17368453 |
| NCBI Gene ID: | 864 |
| NCBI Accession: | Q13761.2 |
| UniProt Secondary Accession: | Q13761,Q12969, Q13760, B1AJV5, |
| UniProt Related Accession: | Q13761 |
| Molecular Weight: | 45,922 Da |
| NCBI Full Name: | Runt-related transcription factor 3 |
| NCBI Synonym Full Names: | runt related transcription factor 3 |
| NCBI Official Symbol: | RUNX3Â Â |
| NCBI Official Synonym Symbols: | AML2; CBFA3; PEBP2aCÂ Â |
| NCBI Protein Information: | runt-related transcription factor 3 |
| UniProt Protein Name: | Runt-related transcription factor 3 |
| UniProt Synonym Protein Names: | Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit |
| Protein Family: | Runt-related transcription factor |
| UniProt Gene Name: | RUNX3Â Â |
| UniProt Entry Name: | RUNX3_HUMAN |
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