The Runx3 Polyclonal Antibody (CAB7303) is a valuable tool for researchers studying the Runx3 protein, a transcription factor known to play a crucial role in cell differentiation and development. This antibody, produced in rabbits, demonstrates high specificity towards human samples and is validated for use in Western blot applications. By targeting the Runx3 protein, this antibody enables precise detection and analysis in various cell types, making it a versatile option for studies in developmental biology, cancer research, and immunology.Runx3, also referred to as runt-related transcription factor 3, is involved in a wide range of biological processes, including cell growth, differentiation, and apoptosis.
Its dysregulation has been linked to various diseases, such as cancer, autoimmune disorders, and developmental abnormalities. Therefore, investigating the function of Runx3 is crucial for understanding these pathologies and potentially identifying new therapeutic targets.With its high specificity and proven utility in Western blotting, the Runx3 Polyclonal Antibody (CAB7303) offers researchers a reliable tool for unraveling the intricate mechanisms mediated by the Runx3 protein in health and disease.
Product Name:
RUNX3 Rabbit Polyclonal Antibody
SKU:
CAB7303
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 286-415 of human RUNX3 (NP_004341.1).
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
864
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse stomach, using RUNX3 antibody (CAB7303) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
