The Runx2 Polyclonal Antibody (CAB2851) is a valuable tool for researchers studying the Runx2 transcription factor, which plays a crucial role in the regulation of skeletal development and osteoblast differentiation. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards Runx2 in human samples, making it ideal for Western blot applications.Runx2, also known as Core-binding factor subunit alpha-1, is essential for the differentiation and maturation of osteoblasts, the bone-forming cells in the body. Dysregulation of Runx2 has been linked to various skeletal disorders, making it a promising target for therapeutic interventions in conditions such as osteoporosis and bone fractures.
By utilizing the Runx2 Polyclonal Antibody, researchers can gain insights into the expression and activity of Runx2 in different cell types and tissues, advancing our understanding of skeletal development and potential treatment strategies for bone-related diseases. Its reliability and specificity make it an indispensable tool for studies in the fields of bone biology, regenerative medicine, and musculoskeletal research.
Product Name:
RUNX2 Rabbit Polyclonal Antibody
SKU:
CAB2851
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 242-521 of human RUNX2 (NP_001019801.3).
This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.
Purification Method:
Affinity purification
Gene ID:
860
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using RUNX2 Rabbit pAb (CAB2851) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
