The CBFA2T1 Polyclonal Antibody (CAB1737) is specifically designed for research involving CBFA2T1, a transcriptional corepressor that plays a key role in hematopoiesis and leukemia. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry. CBFA2T1 is known to interact with a variety of regulatory proteins involved in cell differentiation and proliferation, making it an essential target for studies in cancer research and developmental biology. Its role in transcriptional regulation and chromatin remodeling highlights its importance in understanding gene expression patterns and disease pathogenesis.
By utilizing the CBFA2T1 Polyclonal Antibody, researchers can accurately detect and analyze the expression of CBFA2T1 in different cell types and tissues, providing valuable insights into its function in normal physiology and disease states. This antibody is a valuable tool for investigating the molecular mechanisms underlying hematopoiesis and leukemia, contributing to advancements in precision medicine and targeted therapies for blood-related disorders.
Product Name:
RUNX1T1 Rabbit Polyclonal Antibody
SKU:
CAB1737
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-403 of human RUNX1T1 (NP_783552.1).
This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
862
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of Rat brain, using RUNX1T1 antibody (CAB1737) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
