The Runx1 Polyclonal Antibody (CAB2055) is a valuable tool for researchers studying the Runx1 transcription factor, known for its critical roles in hematopoiesis and development. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot, immunoprecipitation, and immunofluorescence applications.Runx1, also known as AML1, is a key regulator of hematopoietic stem cell differentiation and is frequently implicated in leukemia and other blood disorders. Understanding the function and activity of Runx1 is crucial for unraveling the molecular mechanisms underlying these diseases and identifying potential therapeutic targets.
With its exceptional reactivity and specificity, the Runx1 Polyclonal Antibody enables precise detection and analysis of Runx1 in various cell types, making it an invaluable tool for researchers in the fields of hematology, oncology, and developmental biology. By elucidating the complex regulatory networks involving Runx1, this antibody paves the way for advancements in the diagnosis and treatment of blood-related disorders.
Product Name:
RUNX1/2/3 Rabbit Polyclonal Antibody
SKU:
CAB2055
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 171-260 of human RUNX1 (NP_001001890.1).
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
861
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Molt-4 cells, using RUNX1 Rabbit pAb (CAB2055) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
