The RUNX1 Polyclonal Antibody (CAB0400) is a powerful tool designed for research involving the RUNX1 protein, a key transcription factor involved in hematopoiesis and leukemia. This antibody, produced in rabbits, exhibits high reactivity with human samples and is optimized for use in Western blotting and immunohistochemistry applications. The RUNX1 protein plays a crucial role in the regulation of hematopoietic stem cell differentiation and is often implicated in the development of various leukemia subtypes. The RUNX1 Polyclonal Antibody specifically binds to the RUNX1 protein, allowing for accurate detection and analysis in a variety of cell types and tissues.
With its ability to target and detect the RUNX1 protein, this antibody is an essential tool for researchers in the fields of hematology, oncology, and molecular biology. Understanding the function and regulation of the RUNX1 protein is vital for advancing our knowledge of hematopoiesis and developing targeted therapies for leukemia and other hematologic disorders.
Product Name:
RUNX1/2/3 Rabbit Polyclonal Antibody
SKU:
CAB0400
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human RUNX1 (NP_001001890.1).
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
861
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RUNX1 Rabbit pAb (CAB0400) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
