The Runx1/2/3 Rabbit Monoclonal Antibody (CAB5115) is a versatile tool for researchers studying the transcription factors Runx1, Runx2, and Runx3. These proteins are key regulators of cell differentiation and development, with important roles in hematopoiesis, osteogenesis, and neurogenesis.Raised in rabbits, this antibody offers high specificity and sensitivity in detecting Runx1, Runx2, and Runx3 in various sample types, including human tissues and cell lines. It has been validated for use in applications such as immunofluorescence, immunohistochemistry, and Western blotting, providing reliable results for your experiments.
Runx proteins are known to be dysregulated in a variety of diseases, including leukemia, bone disorders, and developmental abnormalities. By using the Runx1/2/3 Rabbit Monoclonal Antibody, researchers can gain valuable insights into the functions and dysregulation of these transcription factors, leading to a better understanding of disease mechanisms and potential therapeutic targets.
Product Name:
RUNX1/2/3 Rabbit Monoclonal Antibody
SKU:
CAB5115
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 354-453 of human RUNX1/2/3 (NP_001001890.1).
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
861/864/860
Clone Number:
ARC1162
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RUNX1/2/3 Rabbit mAb (CAB5115) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
