The Rufy3 Polyclonal Antibody (CAB12896) offered by AssayGenie is a valuable tool for researchers studying the Rufy3 protein, a key player in cellular processes such as intracellular trafficking and membrane dynamics. This antibody, produced in rabbits, has been rigorously validated for use in various applications, including Western blot and immunofluorescence.Rufy3, also known as RUN and FYVE domain-containing protein 3, is involved in the regulation of cell migration, cytoskeletal organization, and vesicle transport.
Its role in these critical cellular functions makes it an intriguing target for studies in cell biology and cancer research.By using the Rufy3 Polyclonal Antibody, researchers can detect and analyze Rufy3 protein expression in different cell types, providing valuable insights into its function and localization within the cell. This antibody is ideal for investigators seeking to further understand the molecular mechanisms underlying cellular processes and their impact on disease development and progression.
Product Name:
RUFY3 Rabbit Polyclonal Antibody
SKU:
CAB12896
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human RUFY3 (NP_055776.1).
This gene encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
22902
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RUFY3 Rabbit pAb (CAB12896) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
