The Rufy1 Polyclonal Antibody (CAB7836) is a valuable tool for researchers studying Rufy1, a protein involved in cellular processes such as endocytosis and intracellular trafficking. This polyclonal antibody, created by Assay Genie, is highly specific and reactive with human samples, making it ideal for studies in cell biology and cancer research.Rufy1, also known as RUN and FYVE domain containing 1, is known to play a role in the regulation of cell migration and invasion, making it a potential target for therapeutic intervention in cancer metastasis.
This antibody, raised in rabbits, is validated for use in Western blot applications, allowing for the detection and analysis of Rufy1 protein levels in various cell types.By understanding the function and regulation of Rufy1, researchers can gain insights into its role in cancer progression and potentially identify novel targets for drug development. The Rufy1 Polyclonal Antibody is a valuable tool for investigating the mechanisms underlying cellular processes and may pave the way for future therapeutic strategies in cancer treatment.
Product Name:
RUFY1 Rabbit Polyclonal Antibody
SKU:
CAB7836
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 439-708 of human RUFY1 (NP_079434.3).
Cytoplasm, Early endosome membrane, Peripheral membrane protein.
Calculated MW:
80kDa
Observed MW:
80kDa
This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
80230
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RUFY1 antibody (CAB7836) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
