Description
RuBisCO activase Antibody (PACO24900)
The Rubisco Activase Antibody (PAC024900) offered by Assay Genie is a valuable tool for researchers studying the Rubisco activase protein. Rubisco activase is a crucial enzyme involved in the activation of Rubisco, an essential enzyme in the process of carbon fixation during photosynthesis.This polyclonal antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the Rubisco activase protein, researchers can accurately detect and analyze its presence in various cell types.
Research involving Rubisco activase is important in understanding the mechanisms behind photosynthesis and plant growth, making this antibody ideal for studies in plant biology, agriculture, and environmental science. By elucidating the function of Rubisco activase, researchers can potentially enhance crop productivity and develop strategies for mitigating the impact of climate change on plant growth.
Antibody Name: | RuBisCO activase Antibody (PACO24900) |
Antibody SKU: | PACO24900 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
Species Reactivity: | Spinach |
Immunogen: | Ribulose-1,5-bisphosphate carboxylase oxygenase (Native Protein) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot All lanes: Ribulose-1,5-bisphosphate carboxylase oxygenase antibody at 5µg/ml Lane 1: Ribulose-1,5-bisphosphate carboxylase oxygenase at 0.01µg/ml Lane 2: Ribulose-1,5-bisphosphate carboxylase oxygenase at 0.1µg/ml Lane 3: Ribulose-1,5-bisphosphate carboxylase oxygenase at 1µg/ml Lane 4: Ribulose-1,5-bisphosphate carboxylase oxygenase at 10µg/ml Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 55, 14 kDa Observed band size: 55, 14 kDa . |
Background: | Ribulose-1,5-bisphosphate carboxylase oxygenase, most commonly known by the shorter name RuBisCO,is an enzyme involved in the Calvin cycle that catalyzes the first major step of carbon fixation, a process by which the atoms of atmospheric carbon dioxide are made available to organisms in the form of energy-rich molecules such as glucose. RuBisCO catalyzes either the carboxylation or the oxygenation of ribulose-1,5-bisphosphate (also known as RuBP) with carbon dioxide or oxygen. RuBisCO is very important in terms of biological impact because it catalyzes the primary chemical reaction by which inorganic carbon permanently enters the biosphere. Many autotrophic bacteria and archaea fix carbon via the reductive acetyl CoA pathway, the 3-hydroxypropionate cycle or the reverse Krebs cycle, but they make up a relatively minor portion of global net primary production. Phosphoenolpyruvate carboxylase PEPC only temporarily fixes carbon. RuBisCO is also the most abundant protein in leaves, and is considered to be the most abundant protein on Earth.It accounts for 50% of soluble leaf protein in C3 plants (20-30% of total leaf nitrogen) and 30% of soluble leaf protein in C4 plants (5-9% of total leaf nitrogen).Given its important role in the biosphere, there are currently efforts to genetically engineer crop plants so as to contain more efficient RuBisCO. |
Synonyms: | Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic (RA) (RuBisCO activase) |
UniProt Protein Function: | Activation of RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent carboxylation of the epsilon-amino group of lysine leading to a carbamate structure. |
UniProt Protein Details: | |
NCBI Summary: | |
UniProt Code: | P10871 |
NCBI GenInfo Identifier: | 12643998 |
NCBI Gene ID: | |
NCBI Accession: | P10871.2 |
UniProt Secondary Accession: | P10871,Q43327 |
UniProt Related Accession: | P10871 |
Molecular Weight: | 61.78 kDa |
NCBI Full Name: | Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic |
NCBI Synonym Full Names: | |
NCBI Official Symbol: | |
NCBI Official Synonym Symbols: | |
NCBI Protein Information: | |
UniProt Protein Name: | Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic |
UniProt Synonym Protein Names: | |
Protein Family: | Ribulose bisphosphate carboxylase/oxygenase activase |
UniProt Gene Name: | RAÂ Â |
UniProt Entry Name: |