The RTKN Polyclonal Antibody (CAB13349) is a vital tool for researchers studying the Rhotekin protein, a key player in cell signaling and migration processes. This antibody, generated in rabbits, displays high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to RTKN, this antibody enables accurate detection and analysis of the protein in various cell types, making it an ideal choice for studies in cell biology and cancer research.
RTKN, also known as Rho effector, is involved in regulating actin cytoskeleton dynamics and cell morphology, which are crucial for processes such as cell migration and adhesion. Understanding the function of RTKN is essential for unraveling its role in cancer metastasis, cell invasion, and other cellular activities. Research on RTKN may provide valuable insights into the development of targeted therapies for cancer and other diseases characterized by aberrant cell signaling pathways.
Product Name:
RTKN Rabbit Polyclonal Antibody
SKU:
CAB13349
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 254-513 of human RTKN (NP_001015056.1).
This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
6242
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RTKN antibody (CAB13349) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
