The RRAGA/RRAGB Antibody (PAC022551) offered by Assay Genie is a polyclonal antibody designed for research involving RRAGA and RRAGB proteins, key regulators of the mTORC1 signaling pathway. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding to RRAGA and RRAGB proteins, this antibody enables the detection and analysis of these important signaling molecules in various cell types, making it a valuable tool for studies in cell biology and cancer research.RRAGA and RRAGB are small GTPases that function as heterodimers to activate mTORC1, a critical regulator of cell growth and metabolism.
Dysregulation of the mTORC1 signaling pathway has been implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative diseases. Therefore, understanding the role of RRAGA and RRAGB in mTORC1 activation is essential for developing targeted therapies aimed at modulating this pathway in disease contexts.In conclusion, the RRAGA/RRAGB Antibody (PAC022551) from Assay Genie provides researchers with a reliable tool for studying the role of RRAGA and RRAGB proteins in mTORC1 signaling, offering insights into their involvement in various disease processes and potential therapeutic strategies.
Antibody Name:
RRAGA/RRAGB Antibody (PACO22551)
Antibody SKU:
PACO22551
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human RRAGA/B.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HepG2 cells, using RRAGA/B antibody.
Background:
Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
Synonyms:
Ras-related GTP-binding protein A; Rag A; RagA; Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1
UniProt Protein Function:
RRAGA: Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF- alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Can occur as a homodimer, or form a heterodimer with RRAGC or RRAGD in a sequence-independent manner. Binds GTP. The GTP-bound form of RRAGA interacts with NOL8. Interacts with adenovirus E3 14.7 kDa protein. Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain. Belongs to the GTR/RAG GTP-binding protein family.
Molecular Function:GTP binding; GTPase activity; phosphoprotein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; ubiquitin protein ligase binding
Biological Process: apoptosis; cell cycle arrest; cell death; cellular response to amino acid starvation; gene expression; insulin receptor signaling pathway; macroautophagy; negative regulation of autophagy; positive regulation of cytolysis; positive regulation of TOR signaling pathway; transcription initiation from RNA polymerase II promoter; virus-host interaction
