The RPSA Polyclonal Antibody (CAB9008) is specifically designed for research involving regulatory subunit of protein phosphatase 2A (RPSA), a multifunctional protein implicated in various cellular processes. The antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It targets the RPSA protein, allowing for the detection and analysis of RPSA expression in different cell types, making it an ideal tool for studies in cell biology and molecular biology research.
RPSA, also known as 37-kDa laminin receptor precursor (LRP), plays a crucial role in cell adhesion, migration, and survival, making it a significant player in various physiological and pathological processes. Its involvement in cancer progression, metastasis, and drug resistance underscores its importance as a potential therapeutic target. Research into the functions and regulation of RPSA is essential for developing novel treatment strategies and understanding its role in disease development and progression.
Product Name:
67kDa Laminin Receptor Rabbit Polyclonal Antibody
SKU:
CAB9008
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human 67kDa Laminin Receptor (NP_002286.2).
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3921
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit pAb (CAB9008) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
