The RPS3A Polyclonal Antibody (CAB5885) is a valuable tool for researchers studying the RPS3A protein, a key component of the ribosomal subunit involved in protein synthesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the RPS3A protein, this antibody enables precise detection and analysis in various cellular contexts, making it ideal for investigations in molecular biology and cancer research.RPS3A, also known as ribosomal protein S3A, plays a crucial role in ribosome assembly and translation initiation, making it essential for protein production in cells.
Its involvement in these fundamental processes highlights its potential significance in diseases like cancer, where dysregulation of protein synthesis can have dramatic effects on cell growth and survival. Research into the functions of RPS3A could provide valuable insights into the mechanisms underlying cancer development and progression, leading to potential therapeutic interventions targeting this pathway.
Product Name:
RPS3A Rabbit Polyclonal Antibody
SKU:
CAB5885
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6189
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPS3A Rabbit pAb (CAB5885) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
