The RPS27A Polyclonal Antibody (CAB2027) is a valuable tool for researchers studying RPS27A, a key protein involved in ribosome biogenesis and protein synthesis. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding to the RPS27A protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for studies in molecular biology and cancer research.RPS27A, also known as ribosomal protein S27a, is essential for proper ribosome function and protein production in cells. Dysregulation of RPS27A has been implicated in various diseases, including cancer and neurodevelopmental disorders.
By understanding the role of RPS27A in cellular processes, researchers can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets.Overall, the RPS27A Polyclonal Antibody (CAB2027) is a reliable tool for investigating the function of RPS27A in cellular processes and disease states. Its specificity and sensitivity make it a valuable asset for researchers looking to advance their understanding of protein synthesis and its implications in health and disease.
Product Name:
RPS27A Rabbit Polyclonal Antibody
SKU:
CAB2027
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human RPS27A (NP_001170884.1).
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
Purification Method:
Affinity purification
Gene ID:
6233
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293F cells using RPS27A Rabbit pAb(CAB2027) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
