The RPS27A Polyclonal Antibody (CAB14618) is a powerful tool for researchers investigating RPS27A, a key protein involved in protein degradation and cell cycle regulation. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to RPS27A, researchers can accurately detect and analyze this protein in a variety of cell types, making it a valuable asset for studies in molecular biology and cancer research.RPS27A, also known as ubiquitin 40S ribosomal protein S27a, plays a critical role in targeting proteins for degradation and is essential for maintaining cellular homeostasis.
Its involvement in protein turnover and cell division processes makes it a promising target for research into diseases like cancer, neurodegenerative disorders, and metabolic conditions. Understanding the function of RPS27A is crucial for developing therapies that target protein degradation pathways and regulate cellular processes in disease contexts.Overall, the RPS27A Polyclonal Antibody (CAB14618) offers researchers a reliable tool for investigating the role of RPS27A in cellular function and disease pathogenesis, paving the way for new insights and potential therapeutic interventions.
Product Name:
RPS27A Rabbit Polyclonal Antibody
SKU:
CAB14618
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human RPS27A (NP_001170884.1).
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
Purification Method:
Affinity purification
Gene ID:
6233
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPS27A Rabbit pAb (CAB14618) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
