The RPS27 Polyclonal Antibody (CAB6729) offered by Assay Genie is a valuable tool for research involving RPS27, a key protein involved in regulating cellular processes. This antibody, generated in rabbits, has been validated for its high reactivity with human samples and is specifically designed for use in Western blot applications.RPS27, also known as Ribosomal Protein S27, plays a crucial role in protein synthesis and cellular growth. It is involved in maintaining the structural integrity of the ribosome and is essential for proper ribosomal function.
Dysregulation of RPS27 has been implicated in various diseases, including cancer, making it a promising target for further investigation.With the RPS27 Polyclonal Antibody, researchers can accurately detect and analyze RPS27 expression in different cell types, allowing for a better understanding of its role in cellular physiology and disease pathogenesis. By studying the function of RPS27, researchers can potentially uncover novel therapeutic targets for the treatment of cancer and other related disorders.
Product Name:
RPS27 Rabbit Polyclonal Antibody
SKU:
CAB6729
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human RPS27 (NP_001021.1).
cytosol, cytosolic ribosome, nucleoplasm, nucleus, postsynaptic density
Calculated MW:
9kDa
Observed MW:
10kDa
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the S27e family of ribosomal proteins and component of the 40S subunit. The encoded protein contains a C4-type zinc finger domain that can bind to zinc and may bind to nucleic acid. Mutations in this gene have been identified in numerous melanoma patients and in at least one patient with Diamond-Blackfan anemia (DBA). Elevated expression of this gene has been observed in various human cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method:
Affinity purification
Gene ID:
6232
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RPS27 antibody (CAB6729) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
