The RPS10 Polyclonal Antibody (CAB5880) is a valuable tool for researchers studying the ribosomal protein S10 (RPS10), which plays a crucial role in protein synthesis and cell growth. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the RPS10 protein, this antibody allows for the detection and analysis of RPS10 levels in various cell types.RPS10 is a key component of the ribosome, the cellular machinery responsible for translating genetic information into proteins. Dysregulation of RPS10 has been linked to various diseases, including cancer and genetic disorders.
By studying the expression and function of RPS10, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the RPS10 Polyclonal Antibody (CAB5880) offers researchers a reliable tool for investigating the role of RPS10 in cellular processes and disease pathogenesis. Its high specificity and sensitivity make it ideal for use in immunology, cancer research, and other fields where protein synthesis and cell growth are of interest.
Product Name:
RPS10 Rabbit Polyclonal Antibody
SKU:
CAB5880
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human RPS10 (NP_001190174.1).
WB,1:1000 - 1:5000 IHC-P,1:50 - 1:200 IF/ICC,1:100 - 1:500 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
S10; DBA9; eS10; RPS10
Positive Sample:
HeLa,K-562,Mouse liver,Rat thymus
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, nucleolus.
Calculated MW:
19kDa
Observed MW:
19kDa
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).
Purification Method:
Affinity purification
Gene ID:
6204
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPS10 Rabbit pAb (CAB5880) at 1:2000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 30s.
