The RPN2 Polyclonal Antibody (CAB8352) is a valuable tool for research involving RPN2, a protein involved in the regulation of protein folding and quality control in the endoplasmic reticulum. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the RPN2 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential component of studies in cell biology and protein quality control mechanisms.
RPN2, also known as ribophorin II, is essential for the proper folding and glycosylation of proteins in the endoplasmic reticulum, a crucial process for maintaining cellular function and homeostasis. Dysregulation of RPN2 has been implicated in diseases such as cancer and neurodegenerative disorders, highlighting its significance as a potential therapeutic target. By gaining a deeper understanding of the role of RPN2 in protein quality control, researchers can develop treatments that target this pathway to address a variety of diseases.
Product Name:
RPN2 Rabbit Polyclonal Antibody
SKU:
CAB8352
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 360-540 of human RPN2 (NP_002942.2).
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6185
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RPN2 antibody (CAB8352) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
