The RPN1 Polyclonal Antibody (CAB6726) is a valuable tool for researchers studying the RPN1 protein, which is involved in protein degradation processes within cells. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the RPN1 protein, this antibody enables researchers to detect and analyze RPN1 expression in various cell types, making it ideal for investigations in areas such as cell biology and cancer research.RPN1, a subunit of the 26S proteasome, plays a crucial role in the recognition and degradation of misfolded or damaged proteins, thereby maintaining cellular homeostasis.
Dysregulation of the proteasome system, including RPN1, has been implicated in various diseases, making it an important target for therapeutic development. Understanding the function of RPN1 is essential for developing interventions that target protein degradation pathways in conditions such as neurodegenerative disorders, cancer, and autoimmune diseases.
Product Name:
RPN1 Rabbit Polyclonal Antibody
SKU:
CAB6726
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
Endoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein.
Calculated MW:
69kDa
Observed MW:
68kDa
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
Purification Method:
Affinity purification
Gene ID:
6184
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RPN1 antibody (CAB6726) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
