The RPN1 Polyclonal Antibody (CAB12497) is a high-quality research tool designed for studies involving RPN1, a protein associated with protein quality control and ER-associated degradation pathways. Raised in rabbits, this antibody is highly specific to human samples and is validated for use in Western blot applications. By targeting the RPN1 protein, researchers can detect and analyze its expression in various cell types, making it a valuable tool for investigations in molecular biology and protein degradation pathways.RPN1, also known as ribophorin-1, plays a crucial role in the recognition and degradation of misfolded proteins in the endoplasmic reticulum, ensuring proper protein folding and quality control.
Its involvement in protein degradation pathways makes it an important target for research in understanding cellular homeostasis and disease mechanisms. By studying RPN1, researchers can gain insights into protein quality control mechanisms and potential therapeutic targets for diseases related to protein misfolding and degradation.
Product Name:
RPN1 Rabbit Polyclonal Antibody
SKU:
CAB12497
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
Endoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein.
Calculated MW:
69kDa
Observed MW:
68kDa
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
Purification Method:
Affinity purification
Gene ID:
6184
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPN1 Rabbit pAb (CAB12497) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
