RPL7A Antibody (CAB14060)
- SKU:
- CAB14060
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
RPL7A Rabbit Polyclonal Antibody (CAB14060)
The RPL7A Polyclonal Antibody (CAB14060) is a vital tool for researchers studying RPL7A, a ribosomal protein involved in protein synthesis within cells. Produced in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the RPL7A protein, researchers can accurately detect and analyze its expression in various cell types, making it invaluable for studies in molecular biology and cancer research.
RPL7A is essential for ribosome function, playing a crucial role in the translation of genetic information into proteins. Dysregulation of RPL7A has been linked to various diseases, including cancer, making it a promising target for therapeutic interventions. By understanding the function and regulation of RPL7A, researchers can uncover new insights into disease pathology and potentially develop innovative treatments in the future.
| Product Name: | RPL7A Rabbit Polyclonal Antibody |
| SKU: | CAB14060 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-266 of human RPL7A (NP_000963.1). |
| Sequence: | MPKG KKAK GKKV APAP AVVK KQEA KKVV NPLF EKRP KNFG IGQD IQPK RDLT RFVK WPRY IRLQ RQRA ILYK RLKV PPAI NQFT QALD RQTA TQLL KLAH KYRP ETKQ EKKQ RLLA RAEK KAAG KGDV PTKR PPVL RAGV NTVT TLVE NKKA QLVV IAHD VDPI ELVV FLPA LCRK MGVP YCII KGKA RLGR LVHR KTCT TVAF TQVN SEDK GALA KLVE AIRT NYND RYDE IRRH WGGN VLGP KSVA RIAK LEKA KAKE LATK LG |
| Tested Applications: | WB IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 |
| Synonyms: | L7A; eL8; TRUP; SURF3; RPL7A |
| Positive Sample: | A-549,Jurkat,MCF7,Mouse kidney,Rat kidney |
| Conjugate: | Unconjugated |
| Cellular Localization: | cytoplasm, cytosol, cytosolic ribosome, focal adhesion, nucleolus, nucleus |
| Calculated MW: | 30kDa |
| Observed MW: | 35kDa |
Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
| Purification Method: | Affinity purification |
| Gene ID: | 6130 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using RPL7A Rabbit pAb (CAB14060) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s. |
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