The RPL7 Polyclonal Antibody (CAB5932) is a valuable tool for researchers studying RPL7, a ribosomal protein that plays a key role in protein synthesis. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By targeting the RPL7 protein, this antibody allows for precise detection and analysis in various cell types, making it an essential component for studies in molecular biology and cancer research.
RPL7 is essential for ribosome function and overall protein synthesis, making it a crucial player in cell growth and proliferation. Abnormalities in RPL7 expression have been linked to various diseases, including cancer and developmental disorders. By studying the function of RPL7 with this antibody, researchers can gain valuable insights into its role in disease pathogenesis and potentially uncover new therapeutic targets for treatment.
Product Name:
RPL7 Rabbit Polyclonal Antibody
SKU:
CAB5932
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human RPL7 (NP_000962.2).
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method:
Affinity purification
Gene ID:
6129
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPL7 Rabbit pAb (CAB5932) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
