The RPL5 Polyclonal Antibody (CAB1977) is a valuable tool for researchers studying RPL5, a ribosomal protein involved in protein synthesis and cell growth. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the RPL5 protein, this antibody enables accurate detection and analysis of RPL5 in various cell types, making it ideal for investigations in molecular biology and cancer research.RPL5 plays a crucial role in the ribosome assembly process, contributing to the regulation of protein synthesis and cell proliferation.
Dysregulation of RPL5 has been linked to various diseases, including cancer and developmental disorders, highlighting the importance of studying this protein in research. By utilizing the RPL5 Polyclonal Antibody, researchers can gain insights into the function and expression of RPL5, leading to advancements in our understanding of cellular processes and disease mechanisms.
Product Name:
RPL5 Rabbit Polyclonal Antibody
SKU:
CAB1977
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RPL5 (NP_000960.2).
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18P family of ribosomal proteins and component of the 60S subunit. The encoded protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The encoded protein may also function to inhibit tumorigenesis through the activation of downstream tumor suppressors and the downregulation of oncoprotein expression. Mutations in this gene have been identified in patients with Diamond-Blackfan Anemia (DBA). This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome.
Purification Method:
Affinity purification
Gene ID:
6125
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPL5 Rabbit pAb (CAB1977) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
