The RPL35A Polyclonal Antibody (CAB17938) is a valuable tool for researchers studying RPL35A, a ribosomal protein involved in protein synthesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the RPL35A protein, researchers can detect and analyze its expression in various cell types, making it an ideal choice for studies in molecular biology and cancer research.RPL35A, a key component of the ribosome, is essential for the translation of mRNA into proteins.
Dysregulation of RPL35A has been implicated in various diseases, including cancer and genetic disorders. Therefore, investigating the role of RPL35A in protein synthesis and disease pathogenesis is crucial for developing targeted therapies and understanding disease mechanisms.Overall, the RPL35A Polyclonal Antibody (CAB17938) provides researchers with a reliable tool to study the function and expression of RPL35A in different biological contexts, advancing our understanding of protein synthesis and its implications in disease.
Product Name:
RPL35A Rabbit Polyclonal Antibody
SKU:
CAB17938
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 10-90 of human RPL35A (NP_000987.2).
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method:
Affinity purification
Gene ID:
6165
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse stomach, using RPL35A Rabbit pAb (CAB17938) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
