The RPL27A Antibody (PAC023365) is a polyclonal antibody designed for research involving RPL27A, a ribosomal protein that plays a significant role in protein synthesis. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding to the RPL27A protein, researchers can detect and analyze this ribosomal protein in various cell types, making it an ideal tool for studies in molecular biology and cancer research.RPL27A is a crucial component of the ribosome and is essential for the translation of mRNA into protein. Its involvement in protein synthesis makes it a key target for research in understanding cellular processes and disease mechanisms.
By studying the function and expression of RPL27A, researchers can gain insights into various biological processes and potentially identify novel therapeutic targets for diseases such as cancer.Overall, the RPL27A Antibody offers researchers a valuable tool for investigating the role of RPL27A in protein synthesis and its implications in various cellular processes and disease pathways. Its high reactivity and specificity make it a reliable reagent for Western blot analysis, enabling detailed studies into the functions of this important ribosomal protein.
Synthesized peptide derived from C-terminal of human RPL27A.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from COLO cells, using RPL27A antibody.
Immunofluorescence analysis of A549 cells, using RPL27A antibody.
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome.
Synonyms:
MGC87238; RL27A;
UniProt Protein Function:
RPL27A: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008]Protein type: Ribosomal; TranslationChromosomal Location of Human Ortholog: 11p15Cellular Component: cytosol; membraneMolecular Function: protein binding; RNA bindingBiological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
UniProt Protein Details:
NCBI Summary:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008]
