The RPL21 Polyclonal Antibody (CAB14254) is an essential tool for research involving RPL21, a key protein involved in ribosomal protein synthesis. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the RPL21 protein, this antibody enables accurate detection and analysis of RPL21 levels in various cell types, making it an ideal choice for studies focusing on protein synthesis, ribosomal function, and gene expression.RPL21, a ribosomal protein component, plays a crucial role in the assembly of the ribosome and the translation of mRNA into proteins.
Its involvement in ribosomal biogenesis and protein synthesis makes it an intriguing target for research in diverse fields such as molecular biology, cancer biology, and developmental biology. Understanding the functions and regulatory mechanisms of RPL21 can provide valuable insights into the molecular processes underlying cellular growth, proliferation, and differentiation, as well as potential therapeutic strategies for diseases involving dysregulated protein synthesis.
Product Name:
RPL21 Rabbit Polyclonal Antibody
SKU:
CAB14254
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2).
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method:
Affinity purification
Gene ID:
6144
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPL21 Rabbit pAb (CAB14254) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.