The RPL14 Polyclonal Antibody (CAB6724) is a valuable tool for researchers studying RPL14, a protein involved in ribosomal biogenesis and protein synthesis. This antibody, generated in rabbits, exhibits high specificity and reactivity towards human samples, making it suitable for Western blot applications.RPL14, a ribosomal protein, plays a crucial role in the assembly and function of the ribosome, the cellular machinery responsible for protein synthesis. Dysregulation of RPL14 expression has been linked to various diseases, including cancer and developmental disorders, making it a target of interest for research.
By targeting RPL14 with this antibody, researchers can investigate its role in cellular processes and disease pathogenesis.With its ability to detect and quantify RPL14 protein levels in various cell types, the RPL14 Polyclonal Antibody (CAB6724) is an essential tool for studies in molecular biology, cancer biology, and developmental biology. By understanding the function of RPL14, researchers can gain insights into fundamental biological processes and potentially uncover new therapeutic targets for disease intervention.
Product Name:
RPL14 Rabbit Polyclonal Antibody
SKU:
CAB6724
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3).
cytoplasm, cytosol, cytosolic ribosome, extracellular exosome, postsynaptic density
Calculated MW:
23kDa
Observed MW:
26kDa
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method:
Affinity purification
Gene ID:
9045
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RPL14 antibody (CAB6724) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
