The RPL14 Polyclonal Antibody (CAB13384) is a valuable tool for researchers studying RPL14, a ribosomal protein involved in protein synthesis and cell growth. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for various experimental applications, including Western blotting.RPL14 is a fundamental component of the ribosome, the cellular machinery responsible for translating genetic information into functional proteins. Dysregulation of ribosomal proteins like RPL14 has been linked to various diseases, including cancer and developmental disorders.
By targeting RPL14 with this antibody, researchers can investigate its role in these pathological processes and potentially identify novel therapeutic targets.Overall, the RPL14 Polyclonal Antibody provides a valuable tool for studying the function and regulation of RPL14 in different biological contexts, contributing to our understanding of protein synthesis and its implications for human health and disease.
Product Name:
RPL14 Rabbit Polyclonal Antibody
SKU:
CAB13384
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3).
cytoplasm, cytosol, cytosolic ribosome, extracellular exosome, postsynaptic density
Calculated MW:
23kDa
Observed MW:
28kDa
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method:
Affinity purification
Gene ID:
9045
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RPL14 antibody (CAB13384) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
