The RPIA Polyclonal Antibody (CAB8867) is a valuable tool for researchers studying RPIA, a key enzyme involved in the pentose phosphate pathway. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the RPIA protein, this antibody allows for the detection and analysis of RPIA levels in various cell types, making it ideal for research in areas such as metabolism, cancer, and cellular stress response.RPIA plays a crucial role in cellular energy metabolism by catalyzing the conversion of ribose-5-phosphate to ribulose-5-phosphate.
Dysregulation of RPIA has been linked to various diseases, including cancer and metabolic disorders, making it a promising target for therapeutic interventions. By studying the expression and activity of RPIA using this antibody, researchers can gain valuable insights into the role of this enzyme in health and disease, paving the way for novel treatment strategies.
Product Name:
RPIA Rabbit Polyclonal Antibody
SKU:
CAB8867
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 82-311 of human RPIA (NP_653164.2).
B cells,HL-60,Mouse thymus,Rat fat,Mouse spleen,Mouse skeletal muscle,Rat thymus
Conjugate:
Unconjugated
Cellular Localization:
cytosol
Calculated MW:
33kDa
Observed MW:
33kDa
The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18.
Purification Method:
Affinity purification
Gene ID:
22934
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPIA Rabbit pAb (CAB8867) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
