The RPE65 Rabbit Monoclonal Antibody (CAB9615) is a valuable tool for researchers studying retinoid isomerohydrolase RPE65, a key enzyme in the visual cycle within the retina. This antibody is specifically designed to target RPE65 in human samples, making it ideal for Western blot applications. By binding to the RPE65 protein, this antibody allows for accurate detection and analysis in various cell types, facilitating research in ophthalmology and vision science.RPE65 is essential for the conversion of retinyl esters to 11-cis-retinol in the visual cycle, a crucial process for maintaining proper vision.
Dysregulation of RPE65 has been linked to various retinal degenerative diseases, making it a potential therapeutic target for conditions such as retinitis pigmentosa and Leber congenital amaurosis. Understanding the function and regulation of RPE65 is essential for developing novel treatments and interventions for these vision-related disorders.
Product Name:
RPA70 Rabbit Monoclonal Antibody
SKU:
CAB9615
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human RPE65 (Q16518).
The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital.
Purification Method:
Affinity purification
Gene ID:
6121
Clone Number:
ARC1659
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat eye, using RPE65 Rabbit mAb (CAB9615) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
