The RPE65 Polyclonal Antibody (CAB9841) is a valuable tool for researchers studying RPE65, a key protein involved in the visual cycle and essential for normal vision. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it a reliable choice for various research applications, particularly in Western blot analyses.RPE65 is a vital enzyme found in the retina that plays a crucial role in the conversion of vitamin A into the visual pigment rhodopsin, enabling the process of light detection by photoreceptor cells. Mutations in the RPE65 gene can lead to retinal degenerative disorders such as Leber congenital amaurosis and retinitis pigmentosa, highlighting the importance of studying this protein for potential therapeutic interventions.
By utilizing the RPE65 Polyclonal Antibody, researchers can investigate the expression levels and localization of RPE65 in different cell types and tissues, providing insights into its function and potential implications for visual impairment and retinal diseases. This antibody's specificity and sensitivity make it an invaluable tool for advancing our understanding of RPE65 biology and its role in maintaining visual function.
Product Name:
RPA70 Rabbit Polyclonal Antibody
SKU:
CAB9841
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 274-533 of human RPE65 (NP_000320.1).
The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital.
Purification Method:
Affinity purification
Gene ID:
6121
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse eye, using RPE65 Rabbit pAb (CAB9841) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
