RPA70/RPA1 Rabbit Monoclonal Antibody (CAB3367)
- SKU:
- CAB3367
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | RPA70/RPA1 Rabbit Monoclonal Antibody |
SKU: | CAB3367 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Rat |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70/RPA1 (P27694). |
Sequence: | MVGQ LSEG AIAA IMQK GDTN IKPI LQVI NIRP ITTG NSPP RYRL LMSD GLNT LSSF MLAT QLNP LVEE EQLS SNCV CQIH RFIV NTLK DGRR VVIL MELE |
Tested Applications: | WB IP ChIP ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin |
Synonyms: | HSSB; RF-A; RP-A; REPA1; RPA70; MST075; PFBMFT6; RPA70/RPA1 |
Positive Sample: | HeLa,U-251MG,Rat testis |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus, PML body. |
Calculated MW: | 68kDa |
Observed MW: | 70kDa |
This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.
Purification Method: | Affinity purification |
Gene ID: | 6117 |
Clone Number: | ARC0773 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |