The RPA70 Rabbit Monoclonal Antibody (CAB3367) is a valuable tool for researchers studying the role of RPA70 in DNA damage response and repair processes. RPA70, a subunit of the replication protein A complex, plays a crucial role in DNA replication, repair, and recombination.This antibody, generated in rabbits, shows high specificity and sensitivity in detecting RPA70 protein in various cell types and tissues. It is suitable for use in Western blot, immunohistochemistry, and immunofluorescence applications, allowing for detailed analysis and visualization of RPA70 localization and expression levels.
By targeting RPA70, researchers can gain insights into how cells respond to DNA damage and maintain genomic stability. This antibody is a valuable tool for studies investigating cancer, genetic disorders, and other diseases related to DNA damage and repair mechanisms. Its high reactivity and reliability make it a valuable asset for researchers aiming to unravel the complexities of cellular responses to genotoxic stress.
Product Name:
RPA70/RPA1 Rabbit Monoclonal Antibody
SKU:
CAB3367
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70/RPA1 (P27694).
This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.
Purification Method:
Affinity purification
Gene ID:
6117
Clone Number:
ARC0773
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPA70/RPA1 Rabbit mAb (CAB3367) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
