The RPA2 Polyclonal Antibody (CAB2189) is an essential tool for researchers studying replication protein A2 (RPA2), a key player in DNA replication and repair processes. This antibody, generated from rabbit immunizations, exhibits high specificity and sensitivity when detecting RPA2 in human samples, making it a valuable asset in various experimental settings, including Western blot analysis.RPA2 is a critical component of the RPA complex, which plays a crucial role in maintaining genomic stability by safeguarding DNA from damage and ensuring accurate DNA replication. By targeting RPA2, researchers can gain insights into the mechanisms underlying DNA damage response pathways and explore potential therapeutic strategies for conditions associated with DNA damage, such as cancer and genetic disorders.
The RPA2 Polyclonal Antibody is an indispensable tool for investigating the functions and regulatory mechanisms of RPA2 in cellular processes, making it an ideal choice for studies in molecular biology, genomics, and cancer research. Its exceptional performance in detecting RPA2 protein levels makes it a trusted reagent for researchers seeking to unravel the complexities of DNA maintenance and repair mechanisms.
Product Name:
RPA2 Rabbit Polyclonal Antibody
SKU:
CAB2189
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-270 of human RPA2 (NP_002937.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
REPA2; RPA32; RP-A p32; RP-A p34; RPA2
Positive Sample:
293T,293T+UV,NIH/3T3,C6
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, PML body.
Calculated MW:
29kDa
Observed MW:
32kDa
This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.
Purification Method:
Affinity purification
Gene ID:
6118
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPA2 Rabbit pAb (CAB2189) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
