RPA70/RPA1 Rabbit Polyclonal Antibody (CAB0874)
- SKU:
- CAB0874
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | RPA70/RPA1 Rabbit Polyclonal Antibody |
SKU: | CAB0874 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RPA70/RPA70/RPA1 (NP_002936.1). |
Sequence: | MVGQ LSEG AIAA IMQK GDTN IKPI LQVI NIRP ITTG NSPP RYRL LMSD GLNT LSSF MLAT QLNP LVEE EQLS SNCV CQIH RFIV NTLK DGRR VVIL MELE VLKS AEAV GVKI GNPV PYNE GLGQ PQVA PPAP AASP AASS RPQP QNGS SGMG STVS KAYG ASKT FGKA AGPS LSHT SGGT QSKV VPIA SLTP YQSK WTIC ARVT NKSQ IRTW SNSR GEGK LFSL ELVD ESGE IRAT AFNE |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:100 |
Synonyms: | HSSB; RF-A; RP-A; REPA1; RPA70; MST075; PFBMFT6; RPA70/RPA1 |
Positive Sample: | U-87MG,HeLa,293T,A-549 |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus, PML body. |
Calculated MW: | 68kDa |
Observed MW: | 70kDa |
This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.
Purification Method: | Affinity purification |
Gene ID: | 6117 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |