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ROCK2 Polyclonal Antibody (CAB21715)

SKU:
CAB21715
$155
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Description

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Product Name:ROCK2 Polyclonal Antibody
SKU:CAB21715
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1388 of human ROCK2 (NP_004841.2).
Sequence:LDSK DSDI EQLR SQLQ ALHI GLDS SSIG SGPG DAEA DDGF PESR LEGW LSLP VRNN TKKF GWVK KYVI VSSK KILF YDSE QDKE QSNP YMVL DIDK LFHV RPVT QTDV YRAD AKEI PRIF QILY ANEG ESKK EQEF PVEP VGEK SNYI CHKG HEFI PTLY HFPT NCEA CMKP LWHM FKPP PALE CRRC HIKC HKDH MDKK EEII APCK VYYD ISTA KNLL LLAN STEE QQKW VSRL VKKI PKKP PAPD PFAR SSPR TSMK IQQN QSIR RPSR QLAP NKPS
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:ROCK-II; ROCK2
Positive Sample:Human lung
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center.
Calculated MW:161kDa
Observed MW:180kDa

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Purification Method:Affinity purification
Gene ID:9475
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Human lung using ROCK2 Rabbit pAb(CAB21715) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

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