The ROCK1 Monoclonal Antibody (CAB11158) is a vital tool for researchers studying ROCK1, a protein kinase involved in various cellular processes such as cell migration, proliferation, and cytoskeletal dynamics. This antibody, produced through monoclonal antibody technology, demonstrates high specificity and sensitivity in detecting ROCK1 protein in a variety of sample types, including human tissues and cell lines.ROCK1 is a key regulator of actin cytoskeleton organization and cell adhesion, making it a crucial player in cell motility and tissue morphogenesis. Dysregulation of ROCK1 has been linked to cancer metastasis, cardiovascular diseases, and neurological disorders, making it a promising therapeutic target for drug development.
By utilizing the ROCK1 Monoclonal Antibody, researchers can further elucidate the role of ROCK1 in disease progression and potentially identify novel treatment strategies.This antibody is validated for use in Western blotting, immunofluorescence, and immunohistochemistry applications, allowing for versatile detection and visualization of ROCK1 protein expression. Its reliability and consistency make it an essential tool for studies in cell biology, cancer research, and drug discovery aimed at targeting ROCK1 signaling pathways.
Product Name:
ROCK1 Rabbit Monoclonal Antibody
SKU:
CAB11158
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human ROCK1 (Q13464).
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Purification Method:
Affinity purification
Gene ID:
6093
Clone Number:
ARC2371
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using ROCK1 Rabbit mAb (CAB11158) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
