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ROBO3 Rabbit Polyclonal Antibody (CAB14922)

SKU:
CAB14922
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
$167 - $503
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Description

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ROBO3 Rabbit Polyclonal Antibody (CAB14922)

The ROBO3 Polyclonal Antibody (CAB14922) is a valuable tool for researchers studying the ROBO3 protein, a crucial player in axon guidance and neural development. This antibody, derived from rabbit serum, is highly specific to human samples and is validated for use in Western blot and immunohistochemistry applications.ROBO3, a member of the Roundabout (ROBO) family of proteins, is essential for directing neuronal growth cones during development, ensuring proper formation of neural circuits. Dysregulation of ROBO3 has been linked to neurological disorders such as epilepsy and autism spectrum disorders, underscoring its importance in brain function.

By targeting the ROBO3 protein, researchers can explore its role in neural development and disease pathogenesis, paving the way for potential therapeutic interventions. The ROBO3 Polyclonal Antibody provides a reliable tool for investigating the function and regulation of ROBO3 in various experimental settings, offering new insights into neurodevelopmental processes.

Product Name:ROBO3 Rabbit Polyclonal Antibody
SKU:CAB14922
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human ROBO3 (NP_071765.2).
Sequence:MLRY LLKT LLQM NLFA DSLA GDIS NSSE LLLG FNSS LAAL NHTL LPPG DPSL NGSR VGPE DAMP RIVE QPPD LLVS RGEP ATLP CRAE GRPR PNIE WYKN GARV ATVR EDPR AHRL LLPS GALF FPRI VHGR RARP DEGV YTCV ARN
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:HGPS; RIG1; HGPPS; RBIG1; HGPPS1; ROBO3
Positive Sample:Jurkat,SP2/0,SGC-7901
Conjugate:Unconjugated
Cellular Localization:Membrane, Single-pass type I membrane protein.
Calculated MW:148kDa
Observed MW:148kDa

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla.

Purification Method:Affinity purification
Gene ID:64221
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ROBO3 Rabbit pAb (CAB14922) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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