The RNF8 Polyclonal Antibody (CAB7302) is a valuable tool for researchers studying RNF8, a key regulator of DNA damage response and repair processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the RNF8 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for investigations in the fields of DNA repair, genomics, and cancer biology.RNF8 plays a crucial role in orchestrating the recruitment of DNA repair factors to sites of DNA damage, ultimately facilitating the repair of DNA lesions and maintenance of genomic integrity.
Dysregulation of RNF8 has been implicated in various diseases, including cancer, highlighting the importance of understanding its mechanisms of action. By utilizing the RNF8 Polyclonal Antibody, researchers can gain valuable insights into the functions of RNF8 and its implications in disease pathophysiology, potentially paving the way for the development of novel therapeutic strategies targeting DNA repair processes.
Product Name:
RNF8 Polyclonal Antibody
SKU:
CAB7302
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-485 of human RNF8 (NP_003949.1).
The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9025
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using RNF8 antibody (CAB7302) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
