The RNF4 Polyclonal Antibody (CAB12037) is a vital tool for researchers studying the RNF4 protein, a key regulator of various cellular processes. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the RNF4 protein, it enables accurate detection and analysis in a variety of cell types, facilitating research in areas such as cell cycle regulation, DNA damage response, and protein degradation pathways.RNF4, also known as a ubiquitin E3 ligase, plays a crucial role in protein turnover and cellular homeostasis by targeting proteins for ubiquitination and subsequent degradation.
Its involvement in DNA repair and maintenance of genomic stability highlights its importance in cancer biology and neurodegenerative diseases. Investigating the functions of RNF4 can provide insight into disease mechanisms and potential therapeutic targets for these conditions.Overall, the RNF4 Polyclonal Antibody (CAB12037) offers researchers a reliable tool for studying the intricate roles of the RNF4 protein in cellular physiology, making it a valuable asset in the fields of molecular biology, cancer research, and drug discovery.
Product Name:
RNF4 Rabbit Polyclonal Antibody
SKU:
CAB12037
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RNF4 (NP_001171938.1).
The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.
Purification Method:
Affinity purification
Gene ID:
6047
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RNF4 Rabbit pAb (CAB12037) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
