The RNF34 Polyclonal Antibody (CAB8517) is a valuable tool for researchers studying the RNF34 protein, an E3 ubiquitin ligase involved in the regulation of protein degradation and immune responses. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to RNF34, this antibody allows for the detection and analysis of the protein in various cellular contexts, making it an essential component for studies in immunology and cancer research.
RNF34, also known as CARP-1, plays a crucial role in the ubiquitination and degradation of target proteins, including those involved in immune signaling pathways. Its involvement in immune regulation and protein turnover makes it a promising target for research into diseases such as cancer, autoimmune disorders, and inflammatory conditions. Understanding the function and regulation of RNF34 is key to developing novel therapies that target the immune system and protein degradation pathways in these disease contexts.
Product Name:
RNF34 Rabbit Polyclonal Antibody
SKU:
CAB8517
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RNF34 (NP_079402.2).
The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
80196
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RNF34 antibody (CAB8517) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
